Infectious Diseases

An atlas of the clinical microbiology of infectious by Edward J. Bottone

By Edward J. Bottone

content material: v. 1. Bacterial brokers --
v. 2. Viral, fungal, and parasitic agents.

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Extra info for An atlas of the clinical microbiology of infectious diseases. / Volume 1, Bacterial agents

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Pantoea) agglomerans colonies are yellow­pigmented.  Other associations have included brain abscess, thorn­induced eye and wound injuries, and septic arthritis.  vulgaris are strong urease producers.  Four species comprise the genus: S.  Shigellosis is characterized by watery or bloody diarrhea (dysenteric) with mucoid stools. Shigellae are enteroinvasive and can cause necrosis of the colonic epithelium.  Colonies of Salmonella typhi (typhoid bacillus) may appear green without dark centers Page 53 Figure 181 Salmonella species Giemsa stain of bone marrow aspirate of child with AIDS showing intracellular bacilli in macrophage.

Tuberculosis Figure 87 Mycobacterium tuberculosis Apple­green to yellow fluorescing bacilli in sputum smear stained with rhodamine—auramine.  Granules represent individual colonies and are comprised of tightly interwoven bacilli in cord formation Page 33 Morphology The bacilli are either straight or curved to elongated rods, often occurring singly or banded together (cording) because of their lipid­rich hydrophobic surface.  tuberculosis requires enriched media for growth, as provided by Lowenstein­Jensen (egg­based) and Middlebrook 7H10 and 7H11 (serum­based) media.

Malignancies.  Growth has a ‘damp soil’­like odor Figure 126 Mycobacterium fortuitum Ulcerated lesion of leg with sinus tracts which developed post­cosmetic fat transplant to leg.  Because of the reduction in the chain length of their cell wall fatty acid compositionm, these species not only grow more rapidly, but stain weakly Gram­positive and hence can mimic Corynebacterium species (diphtheroids).  Culture of in­use shaving cream was negative Figure 131 Mycobacterium chelonei Smooth colonies growing on 5% sheep blood agar 48 h after touch inoculation of agar with tissue fragments scraped from leg lesion Figure 132 Mycobacterium chelonei Dry, rough, crinkled colonies recovered from broviac catheter segment rolled over agar surface.

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